DESCRIPTION
The product is produced by mixing carefully optimized
proportion of glucoamylase and pullulanase coming from submerged fermentation
of Aspergillus niger and Bacillus subtilis, respectively. The combination of glucoamylase
with supplementary pullulanase makes saccharification more efficient by
avoiding limit dextrin and by-product formation, and therefore saving
processing cost while producing more glucose.
MECHANISM
Pullulanase is a type of isoamylase which can
selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides,
thereby, making the complete hydrolysis of branched starch possible. When used
together with glucoamylase, it speeds up saccharification by degrading α-1,6-glucosidic linkage and increases glucose yield.
REACTION PARAMETERS
PARAMETERS |
RANGE |
Activity Temperature |
30℃-65℃ |
Optimum Temperature |
58℃-64℃ |
Activity pH |
3.5-6.0 |
4.0-4.8 |
PRODUCT SPECIFICATION
Items |
Description |
Declared Activity* |
Glucoamylase
90,000 u/ml Pullulanase 1,000
u/ml |
Physical Form |
Liquid |
Color** |
Brown |
Odour |
Normal microbial fermentation odour. |
*Definition of Unit: 1 unit of Glucoamylase equals to the amount of
enzyme which hydrolyzes soluble starch to get 1mg glucose at 40℃ and pH4.6 in 1h. 1 unit of Pullulanase equals
to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated
as glucose) from pullulan in one minute at pH5.8, 50℃.
** Color: Color can vary from batch to batch. Color
intensity is not an indication of enzyme activity.
PRODUCT STANDARD
The product complies with GB1886.174.
No. |
ITEMS |
INDEX |
|
1 |
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